歯周病のスクリーニングおよびモニタリングのための唾液中のミエロイド関連タンパク質のレベル

Levels of myeloid-related proteins in saliva for screening and monitoring of periodontal disease.

抄録

目的:

歯周病患者の唾液中骨髄関連マーカー濃度とそのスクリーニング能、および歯周病治療がこれらのマーカーに及ぼす影響を評価すること。

AIM: To evaluate the salivary levels of myeloid-related markers in relation to periodontal disease and their potential screening capability, as well as the effects of periodontal treatment on these markers in periodontitis patients.

材料および方法:

健康な歯周組織（n=60）、歯肉炎（n=63）および歯周炎（n=72）の参加者を募集した。歯周炎患者は非外科的治療を受け，3ヶ月と6ヶ月後に再検査を受けた．歯周炎患者には，ベースラインと治療後1，3，6カ月目に無刺激唾液が採取された．Colony-stimulating factor-1（CSF-1），interleukin-34（IL-34），S100A8/A9，S100A12，hepatocyte growth factor（HGF），IL-1β，Matrix metalloproteinase-8（MMP-8） の濃度をイムノアッセイによって分析した．

MATERIALS AND METHODS: Participants with a healthy periodontium (n = 60) and with gingivitis (n = 63) and periodontitis (n = 72) were recruited. Periodontitis patients received non-surgical treatment and were re-examined after 3 and 6 months. Unstimulated saliva was collected at baseline and at 1, 3, and 6 months after therapy for the periodontitis patients. Levels of colony-stimulating factor-1 (CSF-1), interleukin-34 (IL-34), S100A8/A9, S100A12, hepatocyte growth factor (HGF), IL-1β, and matrix metalloproteinase-8 (MMP-8) were analysed by immunoassays.

結果:

歯周炎患者および歯肉炎患者の唾液では、CSF-1、S100A8/A9、S100A12、IL-1β、MMP-8、HGFが健常者と比較して有意に上昇したが、IL-34は健常者、歯肉炎患者のいずれにも比較して、歯周炎で有意に低値であった。IL-34は治療後3カ月で有意に増加し、IL-1βとMMP-8は治療後1カ月で減少した。また、歯周炎患者はS100A8/A9の高値と低値にクラスター化し、高値の患者は出血が多く、ポケットが深く、S100A12が高値であった。

RESULTS: CSF-1, S100A8/A9, S100A12, IL-1β, MMP-8, and HGF were significantly elevated in saliva from periodontitis and gingivitis patients in comparison to healthy individuals, whereas IL-34 was significantly lower in periodontitis compared to both healthy individuals and gingivitis patients. IL-34 increased significantly 3 months after treatment, while IL-1β and MMP-8 decreased 1 month after therapy. Additionally, periodontitis patients clustered in high and low levels of S100A8/A9, whereby those with high levels had more bleeding, deeper pockets, and higher S100A12.

結論:

骨髄関連マーカーの唾液レベルは歯周炎で変化し、歯周治療によって部分的に調節される。唾液中の S100A8/A9 を測定することにより，歯周炎患者の異なるグループを識別できる可能性がある．

CONCLUSIONS: Salivary levels of myeloid-related markers are altered in periodontitis and are partially modulated by periodontal treatment. Measuring S100A8/A9 in saliva may identify distinct groups of periodontitis patients.