fibroblast growth factor-2修飾細胞性皮膚基質膜とウシ由来の異種移植片を用いた歯槽骨隆起の保存。in vivoでの実験的検討

Alveolar ridge preservation with fibroblast growth factor-2 modified acellular dermal matrix membrane and a bovine-derived xenograft. An experimental in vivo study.

• Ruolin Wang
• Wenhua Liu
• Hongmei Guo
• Shaohua Ge
• Haiyun Huang
• Pishan Yang

抄録

目的:

線維芽細胞成長因子2（FGF-2）を添加したバリアー膜を併用した骨補填材が、抜歯後の歯槽骨稜の保存に及ぼす影響を検討すること。

OBJECTIVE: To investigate the effect of a bone substitute material combined with fibroblast growth factor-2 (FGF-2) loaded barrier membrane on the preservation of alveolar ridge after tooth extraction.

材料と方法:

4頭の犬を対象とした。各動物の6つの抜歯窩に3つの治療法を施し、無作為に3つのグループに分けた。A群：ネガティブコントロール、B群：ウシ異種移植片＋メンブレン、C群：ウシ異種移植片＋FGF-2搭載メンブレン。CBCTと組織学的分析を行い、歯槽骨稜の幅と高さの変化、抜歯後8週間の抜歯窩骨癒合を評価した。

MATERIALS AND METHODS: Four dogs were included. Six extraction sockets of each animal received the 3 treatments and were randomly divided into three groups. Group A: negative control; Group B: bovine xenografts + membrane; Group C: bovine xenografts +FGF-2-loaded membrane. CBCT and histological analysis were performed to evaluate changes in the width and height of alveolar ridges and extraction socket bone healing 8 weeks post-extraction.

結果:

CBCTにより、A群の歯槽骨は、B群およびC群に比べて、歯槽堤から1mmおよび3mmの位置で有意に薄くなっていた。C群の1mmにおける歯槽骨の幅（60.99±15.36％）は、B群（39.75±30.18％）よりも有意に厚かった。また、C群の1mmにおける頬側の骨の高さと舌側の骨の幅（87.06±10.34％、89.09±10.56％）は、A群（53.48±23.94％、82.72±12.59％）よりも有意に大きかった。

RESULTS: CBCT showed that the alveolar bone in Group A was significantly thinner than that in Group B and Group C at 1 and 3 mm apically from the alveolar crest. The alveolar width at 1 mm in Group C (60.99 ± 15.36%) was significantly thicker than that in Group B (39.75 ± 30.18%). Histomorphmetrical measurements showed that the buccal alveolar width at 1 mm was significantly thicker in Groups B and C than in Group A. Additionally, buccal bone height and lingual bone width at 1 mm in Group C (87.06 ± 10.34%, 89.09 ± 10.56%) were significantly greater than in Group A (53.48 ± 23.94%, 82.72 ± 12.59%).

おわりに:

今回の結果は、牛骨とFGF-2を含むまたは含まないバリアーメンブレンを歯牙のソケット上に適用することで、特にリッジ幅の吸収を効果的に減少させることができ、FGF-2修飾メンブレンはメンブレンのみで得られる結果を改善するようである。

CONCLUSION: The present findings indicate that application of bovine bone combined with barrier membrane with or without FGF-2 over tooth sockets can effectively reduce ridge absorption, especially in terms of ridge width and FGF-2 modified membrane seems to improve the outcomes obtained with membrane alone.

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