唾液汚染後に埋入された亀裂用シーラントのマイクロリークを防止するためのさまざまなプロトコルのin vitro評価

In Vitro Evaluation of Different Protocols for Preventing Microleakage of Fissure Sealants Placed Following Saliva Contamination.

• Hayrunnisa Şimşek
• A Rüya Yazıcı
• H Cem Güngör

抄録

目的:

エナメル質のコンディショニングプロトコルとその再適用が、唾液汚染後に埋入された亀裂用シーラントのマイクロリークに及ぼす影響を評価する。

PURPOSE: To evaluate the effect of different enamel conditioning protocols and their re-application on the microleakage of fissure sealants placed following saliva contamination.

研究デザイン:

STUDY DESIGN: The study included 156 human third molars in 16 subgroups (2×4×2) under two main groups (sealant type): Group A- hydrophobic resin sealant, 3M Clinpro™ Sealant; Group B- hydrophilic resin sealant, Ultraseal XT Hydro. Each group was then divided according to the type of surface conditioning; 1- Er,Cr:YSSG laser etching, 2- acid-etching, 3- acid-etching+etch-and-rinse adhesive (Prime&Bond® One Select) and 4- self-etching adhesive (Clearfil™ SE Bond). After contaminating the conditioned occlusal enamel surfaces with artificial saliva, fissure sealant was applied in half of the specimens (a), whereas in the other half, (b) the respective surface conditioning was repeated and then fissure sealant was placed. Following thermocycling, the samples were immersed in basic fuchsin, sectioned, and dye penetration was quantitatively assessed with ImageJ. Two-way ANOVA and Bonferroni post-hoc tests were used for statistical analyses (p<0.05).

結果:

A3bとA3aではマイクロリーケージが最も少なく、B4bとB4aではマイクロリーケージが最も多かった。唾液が混入した後，表面処理を再度行わなかった場合，亀裂用シーラントの種類と表面処理の効果は有意であった（それぞれp=0.005，p<0.001）．しかし，それらの相互作用は有意ではなかった（p=0.173）．唾液汚染後に表面処理を再度行った場合，亀裂用シーラントの種類と表面処理の効果（両方ともp=0.000）およびそれらの交互作用（p=0.004）は有意であった．

RESULTS: The least microleakage was observed in A3b and A3a, whereas B4b and B4a were the subgroups with the highest microleakage. Following saliva contamination, when surface conditioning was not re-applied, the effects of fissure sealant types and surface conditioning were significant (p=0.005 and p<0.001, respectively). However, their interaction was insignificant (p=0.173). When surface conditioning was re-applied after saliva contamination, the effects of type of fissure sealant and surface conditioning (p=0.000, for both) and their interaction (p=0.004) were significant.

結論:

3M Clinpro™ SealantはUltraseal XT Hydroよりも優れていた。Er,Cr:YSSGレーザーとセルフエッチング接着剤の再塗布は，両裂孔シーラントのマイクロリークに影響を与えなかった．再塗布しない場合は，アシッドエッチング＋エッチング＆リンス接着剤がアシッドエッチングのみよりも優れていた．しかし，唾液が付着した後に再塗布した場合には，どちらも同様の効果が得られました．

CONCLUSIONS: 3M Clinpro™ Sealant was superior to Ultraseal XT Hydro. Re-application of Er,Cr:YSSG laser and the self-etching adhesive did not affect the microleakage of both fissure sealants. Without re-application, acid-etching+etch-and-rinse adhesive was superior to acid-etching only. However, both of them were similarly successful when they were re-applied following saliva contamination.