塩沼から分離されたShewanella salipaludis sp

Description of Shewanella salipaludis sp. nov., isolated from a salt marsh.

• Sooyeon Park
• Siyu Chen
• Jung-Sook Lee
• Wonyong Kim
• Jung-Hoon Yoon

抄録

韓国黄海の塩沼からグラム染色陰性の鞭毛菌SHSM-M6T株を分離した。16S rRNA遺伝子配列の近傍結合系統樹から、SHSM-M6T株はシェワネラ属に属することが明らかになった。SHSM-M6T株とシェワネラ属の型株との16S rRNA遺伝子配列の類似度は98.0%以下であった。また，SHSM-M6T株のゲノム配列とシェワネラ属の型株との間のANI値は73.3以下，dDDH値は20.7％以下であった。SHSM-M6T株は、メナキノンとしてMK-6を、ユビキノンとしてQ-7およびQ-8を含有していた。また、主要脂肪酸としてC16:1ω7cおよび/またはC16:1ω6c、iso-C15:0およびC16:0を含有していた。株SHSM-M6Tの主な極性脂質は、ホスファチジルエタノールアミン、ホスファチジルグリセロール、未同定脂質1種、未同定アミノ脂質1種、未同定リン脂質1種であった。SHSM-M6T株の表現型特性の違い、およびその系統的および遺伝的特徴から、SHSM-M6T株は認識されているShewanella種から分離されていることが明らかになった。これらの結果から、SHSM-M6T株はシェワネラ属の新種であると考えられ、Shewanella salipaludis sp.型はSHSM-M6T ( = KACC 19901T=NBRC 113646T)である。

A Gram-stain-negative and flagellated bacterial strain, SHSM-M6T, was isolated from salt marsh from Yellow Sea, Republic of Korea. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain SHSM-M6T belongs to the genus Shewanella. 16S rRNA gene sequence similarity values between strain SHSM-M6T and the type strains of Shewanella species were less than 98.0%. The ANI and dDDH values between genomic sequences of strain SHSM-M6T and the type strains of Shewanella species were less than 73.3 and 20.7%, respectively. Strain SHSM-M6T contained MK-6 as predominant menaquinone and Q-7 and Q-8 as the predominant ubiquinones. The novel strain contained C16:1ω7c and/or C16:1ω6c, iso-C15:0 and C16:0 as major fatty acids. Major polar lipids of strain SHSM-M6T were phosphatidylethanolamine, phosphatidylglycerol, one unidentified lipid, one unidentified aminolipid and one unidentified phospholipid. Differential phenotypic properties of strain SHSM-M6T, together with its phylogenetic and genetic distinctiveness, revealed that strain SHSM-M6T is separated from recognized Shewanella species. On the basis of the data presented, strain SHSM-M6T is considered to represent a novel species of the genus Shewanella, for which the name Shewanella salipaludis sp. nov. is proposed. The type strain is SHSM-M6T ( = KACC 19901T = NBRC 113646T).

© FEMS 2020.