糖尿病ラットの皮膚創傷治癒に対するプロバイオティクス補給の効果

EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS.

• Letícia Fuganti Campos
• Eliane Tagliari
• Thais Andrade Costa Casagrande
• Lúcia de Noronha
• Antônio Carlos L Campos
• Jorge Eduardo F Matias

抄録

背景:

での創傷収縮部の例。A)対照群の手術日、B)対照群の7PO、C)プロバイオティクス群の手術日、D)プロバイオティクス群の7PO。糖尿病患者の慢性的な創傷は、しばしば炎症性サイトカインの長期にわたる過剰な産生のために不治の病となる。プロバイオティクスの使用は、腸内マイクロバイオータを改変し、炎症反応を変調させる。

BACKGROUND: Example of wound contraction area at: A) day of surgery in the control group; B) 7PO in the control group; C) day of surgery in the probiotic group; D) 7PO in probiotic group. Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions.

目的:

糖尿病ラットの皮膚治癒過程におけるプロバイオティクスの周術期補給の影響を評価する。

AIM: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats.

方法:

METHODS: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E.

結果:

P10群では創部の収縮が早く、その結果、瘢痕面積が小さくなった（p=0.011）。プロバイオティクス群では術後3日目から10日目にかけてI型コラーゲンの沈着が増加していた（p=0.016）が、対照群では増加していなかった（p=0.487）。組織学的解析では、多形核が少なく（p<0.001）、新生血管が多い（p=0.001）P10群の方が治癒度が高かった（p=0.005）。

RESULTS: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001).

結論:

プロバイオティクスの周術期補給は糖尿病ラットの皮膚創傷治癒を刺激し、炎症反応の減衰と新生血管化とI型コラーゲン沈着の増加に起因する可能性がある。

CONCLUSIONS: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.