中国漢民族集団におけるS100B 3'UTR多型と慢性心不全リスクとの関連

Association of S100B 3'UTR polymorphism with risk of chronic heart failure in a Chinese Han population.

• Yuewu Chen
• Xianghong Chen
• Maozhong Yao
• Lei Chen
• Weiwei Chen
• Xianxia Liu

抄録

中国漢民族集団における S100B の 3'非翻訳領域（UTR）rs9722 遺伝子座の一塩基多型（SNP）と慢性心不全（CHF）リスク、S100B タンパク質および has-miR-340-3p の血漿レベルとの相関を検討した。虚血性心筋症（ICM）124例、拡張型心筋症（DCM）91例の合計215例のCHF患者と215例の健常対照者をリクルートし、S100B rs9722遺伝子型をサンガーシークエンシングで解析した。血漿中のhsa-miR-340-3pのレベルをRT-PCRで検出し、S100BのレベルをELISAで検出した。S100B rs9722遺伝子座T対立遺伝子保持者のCHFリスクは、C対立遺伝子保持者のCHFリスクの4.24倍であった（95％CI：2.84-6.33、P"Zs_200A"<"Zs_200A".001）。S100B rs9722遺伝子座SNPとICMおよびDCMリスクとの関連は、年齢、性別、ボディマスインデックス（BMI）などの因子に影響されなかった。ICMおよびDCM患者の血漿S100Bおよびhsa-miR-340-3pのレベルは、対照群と比較して有意に高かった（P"Zs_200A"<"Zs_200A".001）。ICM患者とDCM患者の間では、血漿S100B濃度に有意差はなかった（P"Zs_200A">"Zs_200A".05）。ICM、DCMおよび対照群の中で、TT遺伝子型キャリアは血漿S100Bおよびhsa-miR-340-340-3pのレベルが最も高く、次いでCT遺伝子型およびTT遺伝子型であり、その差は統計的に有意であった(P"Zs_200A"<"Zs_200A".05)。血漿中のhsa-miR-340-340-3pレベルは、対照群およびICMおよびDCM患者において、S100Bレベルと正の相関を示した。

To study the correlation between single nucleotide polymorphism (SNP) of the 3' untranslated region (UTR) rs9722 locus in S100B and the risk of chronic heart failure (CHF), plasma levels of S100B protein as well as has-miR-340-3p in a Chinese Han population.A total of 215 patients with CHF (124 ischemic cardiomyopathy (ICM) and 91 dilated cardiomyopathy (DCM)) and 215 healthy controls were recruited to analyze the S100B rs9722 genotype by Sanger sequencing. The levels of hsa-miR-340-3p in the plasma were detected by RT-PCR, and S100B levels were detected by ELISA.The risk of CHF in S100B rs9722 locus T allele carriers was 4.24 times higher than that in those with the C allele (95% CI: 2.84-6.33, P < .001). The association of S100B rs9722 locus SNP with ICM and DCM risk was not affected by factors such as age, gender, and body mass index (BMI). The levels of plasma S100B and hsa-miR-340-3p in patients with ICM and DCM were significantly higher than those in the control group (P < .001). There was no significant difference in plasma S100B levels between patients with ICM and DCM (P > .05). Among ICM, DCM, and control subjects, TT genotype carriers had the highest levels of plasma S100B and hsa-miR-340-3p, followed by the CT genotype and TT genotype, and the difference was statistically significant (P < .05). Plasma hsa-miR-340-3p levels were positively correlated with S100B levels in the control subjects and patients with ICM and DCM.The S100B rs9722 locus SNP is associated with CHF risk in a Chinese Han population.