磁場を用いた細胞の抗アポトーシス効果

Anti-apoptotic effect of a static magnetic field in human cells that had been treated with sodium fluoride.

• Magdalena Kimsa-Dudek
• Agnieszka Synowiec-Wojtarowicz
• Agata Krawczyk
• Celina Kruszniewska-Rajs
• Stanisław Gawron
• Monika Paul-Samojedny
• Joanna Gola

抄録

静磁場(SMF)は産業界、民生用機器、診断用医療機器などで広く使用されており、自然環境や職業的にSMFにさらされている人々の間では、SMFへの曝露が広く行われています。環境や一部の職場では、様々な化学物質への曝露の危険性もある。そこで、本研究の目的は、フッ化物イオンと共処理したヒト線維芽細胞のアポトーシス関連遺伝子の発現に及ぼすSMFの影響を評価することであった。フローサイトメトリー分析の結果、フッ化物イオンは生存細胞数を減少させ、初期のアポトーシスを誘導することがわかった。しかし、ＳＭＦへの曝露は、フッ化物イオンで処理した死細胞の数を減少させた。また、アポトーシスに関与する特定の遺伝子の発現には差があり、SMFに曝露することでその転写活性が調節されることがわかった。

Static magnetic field (SMF) is widely used in industry, in consumer devices and diagnostic medical equipment, hence the widespread exposure to SMF in the natural environment and in people occupationally exposed to it. In environment and in some workplaces, there is a risk of exposure also to various chemicals. Environmental factors can affect the cellular processes which can be the cause of the development of various pathological conditions.Therefore, the aim of this study was to assess the effect of SMF on the expression of the apoptosis-related genes in human fibroblast cultures that had been co-treated with fluoride ions.The control and NaF-treated cells were subjected to the influence of SMF with a moderate induction. The flow-cytometric analysis showed that the fluoride ions reduced the number of viable cells and induced early apoptosis. However, exposure to the SMF reduced the number of dead cells that had been treated with fluoride ions. Moreover, specific genes that were involved in apoptosis exhibited a differential expression in the NaF-treated cells and exposure to the SMF yielded a modulation of their transcriptional activity.Our results suggest some beneficial properties of using a moderate-intensity static magnetic field to reduce the adverse effects of fluoride.