アルギニンを含有するフッ化物ワニスのエナメル質再石灰化能の検討

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J Dent.2020 Jun;:103411. S0300-5712(20)30157-3. doi: 10.1016/j.jdent.2020.103411.Epub 2020-06-22.

アルギニンを含有するフッ化物ワニスのエナメル質再石灰化能の検討

The enamel remineralization potential of fluoride varnishes containing arginine.

Mohammed Nadeem Bijle
Manikandan Ekambaram
Edward Cm Lo
Cynthia Kar Yung Yiu

PMID: 32585260 DOI: 10.1016/j.jdent.2020.103411.

抄録

目的:

5%フッ化ナトリウム(NaF)ワニスにアルギニンを配合した場合の再石灰化能とHGF-1細胞毒性への影響を検討する。

OBJECTIVES: To examine the effect of incorporating arginine in a 5% sodium fluoride (NaF) varnish on its remineralization potential and HGF-1 cytotoxicity.

方法:

METHODS: Experimental varnishes were prepared using two arginine variants: L-arginine (Arg) and L-arginine monohydrochloride (Arg.HCl) at 2%, 4%, & 8% w/v. with control 5% NaF varnish. Sound enamel specimens with varnish treatments were subjected to a 4-day demineralization assay and surface roughness analysis using AFM (n = 3). Enamel specimens with artificial incipient caries-like lesions were subjected to a remineralization assay (n = 6) and an 8-day pH-cycling (n = 3). For remineralization assay, enamel F/Arg-uptake and Ca/POi-content were estimated. During pH-cycling, EDX mapping for Ca/F-content and mineral density using micro-CT were assessed. The HGF-1 cytotoxicity of the varnishes was examined using MTT/CCK-8 assay.

結果:

2%Arg-NaFワニス処理した試料のCa含有量/F取り込み量は他のワニスと比較して有意に高かった(p<0.05)。2%Arg-NaFはNaFに比べて有意に高い再石灰化能を示し(p<0.001)，ワニス塗布後にArg-Ca-F複合体が形成されていることが示唆された．8%Arg-NaFは2%Arg-NaF(p<0.001)とNaFワニスに比べて、HGF-1細胞に対して有意に顕著な細胞毒性を示した。

RESULTS: The Ca-content/F-uptake of the 2% Arg-NaF varnish treated specimens were significantly higher than other varnishes (p < 0.05). The 2% Arg-NaF had significantly higher remineralization potential than NaF (p < 0.001), suggesting Arg-Ca-F complex formation post-varnish application. The 8% Arg-NaF exhibited significantly more pronounced cytotoxicity to HGF-1 cells than 2% Arg-NaF (p < 0.001) and NaF varnish.

結論:

2%ArgをNaFワニスに添加するとエナメル質再石灰化能が向上し、8%Argを添加したNaFワニスはHGF-1に対して細胞毒性を示した。

CONCLUSIONS: Incorporation of 2% Arg in NaF varnish enhanced the enamel remineralization potential of NaF varnish; while 8% Arg in NaF varnish was cytotoxic to HGF-1.

臨床的意義:

虫歯予防のためのL-アルギニンを濃縮したNaFワニスは、世界的な公衆衛生上の優先事項に対応しています。L-アルギニンとNaFワニスの相乗効果は、フッ化物の限られた抗菌効果に対抗し、バランスのとれた口腔内マイクロバイオームの獲得をサポートします。アルギニンとフッ化物を組み合わせたワニスは、う蝕予防のために必要とされる生態学的アプローチを提供します。

CLINICAL SIGNIFICANCE: A professionally deliverable L-arginine-enriched NaF varnish for caries prevention addresses a global public health priority. The synergism between L-arginine and NaF varnish counters the limited antimicrobial effects of fluorides and supports acquisition of a balanced oral microbiome. The combined arginine-fluoride varnish will provide the much-needed ecological-based approach for caries prevention.