中国メリノシープ（ユンケン型）の銅欠乏自然放牧地での反応について

Responses of Chinese Merino Sheep (Junken Type) on Copper-Deprived Natural Pasture.

• Xiaoyun Shen
• Chunjie Song

抄録

中国メリノシープ（ユンケン型）の銅欠乏天然放牧地での反応を研究するために、土壌、飼料、羊組織中のミネラル含有量を分析した。また、生理学的・生化学的パラメータも測定した。その結果、影響を受けた牧草地の土壌および飼料中の銅濃度は、健康な範囲の銅濃度に比べて有意に低いことが示された（P＜0.01）。また、被害を受けた中国メリノシープの動物組織（血液、肝臓、羊毛）中の銅含有量も健常羊に比べて有意に低かった（P<0.01）。罹患した中国メリノシープのヘモグロビン値、パックセル体積、平均コルプス体積、平均コルプスセルヘモグロビン量は健常者より有意に低かった(P<0.01)。血清中のセルロプラスミンは健常者に比べて有意に低く、血清中の乳酸脱水素酵素、アルカリホスファターゼ、アスパラギン酸アミノトランスフェラーゼ、アラニンアミノトランスフェラーゼの活性は健常者に比べて有意に高かった(P<0.01)。血清中のスーパーオキシドジスムターゼ、グルタチオンペルオキシダーゼ、総抗酸化能、カタラーゼは健常羊に比べて有意に低く、マロンジアルデヒド含量は健常羊に比べて有意に高かった(P<0.01)。銅欠乏羊のインターロイキン-2、インターロイキン6、インターロイキン-1β、免疫グロブリンA、免疫グロブリンM、免疫グロブリンGのレベルは健常羊に比べて有意に低かった(P<0.01)。飼料中の銅欠乏は血中ミネラル含量に影響を与えるだけでなく、中国メリノシープの血液パラメータ（生理、生化学、免疫、抗酸化）にも深刻な障害を与えた。

To research responses of Chinese Merino Sheep (Junken type) to copper-deprived natural pasture, we analyzed mineral contents in soil, forage, and sheep tissues. Physiological and biochemical parameters were also determined. Results showed that copper concentrations in soil and forage from affected pastures were significantly lower than those in healthy ranges (P < 0.01). Copper contents in animal tissues (blood, liver, and wool) from affected Chinese Merino Sheep were also significantly lower than those in healthy sheep (P < 0.01). Hemoglobin levels, packed cell volume, mean corpuscular volume, and mean corpuscular hemoglobin from affected Chinese Merino Sheep were significantly lower than those in healthy animals (P < 0.01). Serum ceruloplasmin was significantly lower in affected Chinese Merino Sheep than that in healthy animals, while activities of lactate dehydrogenase, alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase in serum were significantly higher in affected compared with healthy animals (P < 0.01). The levels of superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and catalase in serum were significantly lower, and the malondialdehyde content was significantly higher in affected compared with healthy sheep (P < 0.01). The levels of interleukin-2, interleukin 6, interleukin-1β, immunoglobulin A, immunoglobulin M, and immunoglobulin G in copper-deprived sheep were significantly lower than those in healthy animals (P < 0.01). Copper deprivation in forage not only influenced the mineral content in blood but also severely disrupted blood parameters (physiology, biochemistry, immunity, and antioxidant) in Chinese Merino Sheep.