パエオニーの全グルコシド（TGP）は、シェーグレン症候群を誘発したマウスの便秘と腸の炎症を緩和する

Total glucosides of paeony (TGP) alleviates constipation and intestinal inflammation in mice induced by Sjögren's syndrome.

• Ge Liu
• Ziyu Wang
• Xiang Li
• Rui Liu
• Binbin Li
• Liangliang Huang
• Yan Chen
• Chongxi Zhang
• Honghao Zhang
• Yunman Li
• Yongjian Chen
• Hong Yin
• Weirong Fang

抄録

文化人類学的変化:

シェーグレン症候群（SS）は自己免疫疾患であり、便秘や腸の炎症などの胃腸障害を引き起こすことがあります。薬用素材の一種として、Paeonia lactiflora Pallは様々な薬理作用を持ち、多くの医薬品調剤にも欠かせない成分であり、医学界、薬学界から広く関心を持たれています。パエオニーの全グルコシド（TGP）は、Paeonia lactiflora Pallの根から抽出された生理活性化合物の混合物であり、様々な自己免疫疾患に対する治療効果を有している。

ETHNOPHARMACOLOGICAL RELEVANCE: Sjögren's syndrome (SS) is an autoimmune disease and can cause gastrointestinal disorders such as constipation and intestinal inflammation. As a kind of medicinal material, Paeonia lactiflora Pall has a variety of pharmacological effects, and it is also an indispensable component in many pharmaceutical preparations, which has been widely concerned by the medical and pharmaceutical circles. Total glucosides of paeony (TGP) is a mixture of biologically active compounds extracted from the root of Paeonia lactiflora Pall and has therapeutic effects on a variety of autoimmune diseases.

研究の目的:

SSをモデルにしたマウスの便秘や腸内炎症に対するTGPの治療効果を調べ、臨床研究の基礎とする。

AIM OF THE STUDY: To investigate the therapeutic effect of TGP on constipation and intestinal inflammation in mice modeled by SS, and to provide a basis for clinical research.

材料と方法:

MATERIALS AND METHODS: The SS model was set up by submandibular gland (SMG) immune induction method and then treated with TGP for 24 weeks. The fecal characteristics were observed and the fecal number and moisture content were measured. Colonic pathology was observed by H&E staining. The levels of serum P substance (SP), vasoactive intestinal peptide (VIP), interleukin (IL)-1β, tumor necrosis factor (TNF)-α, nuclear factor (NF)-κB, nitric oxide (NO), and nitric oxide synthase (NOS) were determined by enzyme linked immunosorbent assay (ELISA) and microplate method, respectively. Reverse transcription polymerase chain reaction (RT-PCR) was employed to analyze the mRNA expression of c-kit and stem cell factor (SCF) in colon.

結果:

TGPカプセル投与により、モデル群と比較して、便の乾燥・荒れが改善され、便の光沢、数、含水率が有意に増加しました。その間、TGP の処置は大腸の病理学的な損傷を改善し、NO、NOS、IL-1β、TNF-α、NF-κB および SP の血清濃度を抑制し、血清 VIP の集中を高め、大腸の SCF および c-kit の mRNA の発現をアップレギュレートしました。

RESULTS: Compared with the model group, the dry and rough condition of the feces was improved, and the fecal gloss, number and moisture content significantly increased after the administration of TGP capsules. Meanwhile, TGP treatment improved colonic pathological damage, inhibited the serum concentrations of NO, NOS, IL-1β, TNF-α, NF-κB and SP, increased serum VIP concentration, and up-regulated mRNA expression of SCF and c-kit in colon.

結論:

TGPは、腸管運動関連因子や炎症因子に作用することで、マウスのSSによる便秘や腸内炎症を明らかに減衰させることができると考えられます。

CONCLUSIONS: TGP could obviously attenuate SS-mediated constipation and intestinal inflammation in mice by acting on some intestinal motility related factors and inflammatory factors.

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