カルシウム依存性および非依存性アネキシンVの結合：細胞膜界面での異なる分子挙動

Calcium-dependent and -independent annexin V binding: distinct molecular behaviours at cell membrane interfaces.

• Yong-Hao Ma
• Bolin Li
• Jingjing Yang
• Xiaofeng Han
• Zhan Chen
• Xiaolin Lu

抄録

Ca2+依存型と非依存型のアネキシンVの結合挙動を和周波発生振動分光法を用いて比較検討した。その結果、Ca2+非依存的な結合は、Ca2+を添加するとアネキシンVの配向が変化し、より安定な状態へと変化することがわかった。

Distinct annexin V binding behaviours in Ca2+-dependent and Ca2+-independent cases were comparatively investigated using sum frequency generation vibrational spectroscopy. It was discovered that binding affected the molecular arrangement of both membrane leaflets, and the initial Ca2+-independent binding went through a transition with annexin V reorientation to a more stable state upon adding Ca2+.