歯周病患者の腸内細菌叢の定義：探索的研究

Defining the gut microbiota in individuals with periodontal diseases: an exploratory study.

• Talita Gomes Baeta Lourenςo
• Sarah J Spencer
• Eric John Alm
• Ana Paula Vieira Colombo

抄録

:歯周病患者の腸内細菌叢の特徴を明らかにし，歯周病の炎症や組織破壊との関連を明らかにすることを目的とした。 歯周病患者（PH=7名），歯肉炎患者（=14名），慢性歯周炎患者（CP=23名）から便を採取し，Illumina MiSeq法により腸内細菌叢を解析した。歯周病患者では、歯肉炎患者と比較して、α値が増加し、一方、歯周病患者では減少した。 (genus)、(family)、(order)は、Gでは多く検出されたが、PHでは(order)が優勢であった(<0.01)。歯周病菌を代表するOTUとアタッチメントロスの間には有意な相関関係（rho=0.337-0.468, <0.01）が認められ，歯周病患者とPHを識別することができた（全体の精度=84％）．歯周病患者の腸内細菌叢は多様性に欠けており、全身性の炎症性疾患と一致していた。歯周病患者の腸内細菌叢には、歯周病の状態に関わらず、歯周病の破壊や炎症に関連する口腔内細菌が多く検出されました。

: This exploratory study aimed to characterize the gut microbiome of individuals with different periodontal conditions, and correlate it with periodontal inflammation and tissue destruction. : Stool samples were obtained from individuals presenting periodontal health (PH = 7), gingivitis ( = 14) and chronic periodontitis (CP = 23). The intestinal microbiome composition was determined by Illumina MiSeq sequencing. : A lower alpha-diversity in the gut microbiome of individuals with CP was observed, although no significant difference among groups was found ( > 0.01). and were increased, whereas were decreased in abundance in patients with periodontitis compared to PH. (genus), (family) and (order) were detected in higher numbers in G, while (order) was predominant in PH ( < 0.01). Significant correlations (rho = 0.337-0.468,  < 0.01) were found between OTUs representative of periodontal pathogens and attachment loss. and were able to discriminate individuals with periodontal diseases from PH (overall accuracy = 84%). Oral taxa were detected in high numbers in all stool samples. : Individuals with periodontal diseases present a less diverse gut microbiome consistent with other systemic inflammatory diseases. High numbers of oral taxa related to periodontal destruction and inflammation were detected in the gut microbiome of individuals regardless of periodontal status.