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低出生体重児妊娠マウスの胎盤および臍帯におけるPorphyromonas gingivalis感染症の影響
Effect of Porphyromonas gingivalis infection in the placenta and umbilical cord in pregnant mice with low birth weight.
PMID: 29334319 DOI: 10.1080/00016357.2018.1426876.
抄録
目的:
歯周炎と分娩転帰との関連性を示すエビデンスが増えているが，そのメカニズムは不明である．本研究では、マウスにおけるPorphyromonas gingivalis（Pg）感染が分娩成績に及ぼす影響を検討することを目的とした。
OBJECTIVE: Growing evidence indicates an association between periodontitis and delivery outcome; however, the mechanism is unclear. This study aimed to investigate the influence of Porphyromonas gingivalis (Pg) infection on delivery outcome in mice.
材料および方法:
妊娠中のSlc:ICRマウス（8週齢）にPgを静脈内注射して菌血症を誘導し、対照群（CO）と妊娠1日目（GD1）、妊娠15日目（GD15）、毎日（ED）にPgを注射する群に無作為に分けた。分娩転帰、Pg感染、胎盤と臍帯における遺伝子発現を評価した。
MATERIALS AND METHODS: Bacteremia was induced in pregnant Slc:ICR mice (8 weeks old) by intravenous injection of Pg. Mice were randomly divided into a control group (CO), and those receiving Pg injection at gestational day 1 (GD1), gestational day 15 (GD15) or every day (ED). Delivery outcome, Pg infection, and gene expression in the placenta and umbilical cord were evaluated.
結果:
出生体重は、ED群とGD15群でCO群に比べて低かった。胎盤や臍帯ではPgは検出されなかったが、抗Pg IgG抗体の増加がED群とGD1群で顕著に認められ、胎盤ではTnfαとIl6、臍帯ではHif1αのmRNA発現がED群で有意に増加した。臍帯のマイクロアレイ解析では、ED群でOrm1、Mgl2、Rps6ka3、Trim15を含むいくつかの遺伝子の発現が増加していた。
RESULTS: Birth weight was lower in the ED and GD15 groups than in the CO group. A remarkable increase in anti-Pg IgG antibody was observed in the ED and GD1 groups, although Pg was not detected in the placenta or umbilical cord. mRNA expression of Tnfα and Il6 in the placenta, and Hif1α in the umbilical cord, was significantly increased in the ED group. Microarray analysis of the umbilical cord revealed increased expression of several genes including Orm1, Mgl2, Rps6ka3 and Trim15 in the ED group.
結論:
第3期のPg感染で低出生体重、胎盤や臍帯に炎症が起きた。
CONCLUSIONS: Pg infection during the third trimester caused low birth weight and inflammation in the placenta and umbilical cord.