脱灰した根面へのレジン浸潤の評価。インビトロ試験の結果

Evaluation of resin infiltration on demineralized root surface: An in vitro study.

抄録

本研究の目的は，Streptococcus mutansのバイオフィルムによって誘発された根面齲蝕に対するレジン浸潤の効果を評価することである．ヒト小臼歯試料を，ネガティブコントロール（NC），Clearfil SE Bond（SEB），Icon-etch120s+Icon-filtrant（HA120），Icon-etch10s+Icon-filtrant（HA10），K-etchant10s+Icon-filtrant（PA10）の5群に分けた．レジンの浸透状況は蛍光顕微鏡で観察した。バイオフィルムによる脱灰を再度行い，掃引光源型光干渉断層計と共焦点レーザー走査型顕微鏡で観察した。最大樹脂浸透深さ（PDmax），病巣深さ増加量（△LD），歯頸部エナメル質の脱落頻度，歯エナメル質接合部分離長を測定し，統計的に解析した。HA120は138.00±49.25 µmのPDmaxを示し，PA10やSEBよりも有意に大きかった（p<0.05）．SEBは136.58±64.73µmのコーティング層を形成した。HA120とSEBはNCよりも有意に低い∆LDを示した(p<0.05)。120s-HCl前処理によるレジン浸潤は，良好な浸潤能力と根面齲蝕予防効果を有するが，歯頸部エナメル質喪失の追加的な危険因子が確認された．

The aim of this study was to evaluate the effect of resin infiltration on root caries induced by Streptococcus mutans biofilms. Human premolar specimens were divided to 5 groups: negative control (NC), Clearfil SE Bond (SEB), Icon-etch120s+Icon-infiltrant (HA120), Icon-etch10s+Icon-infiltrant (HA10) and K-etchant10s+Icon-infiltrant (PA10). The resin penetration was observed by fluorescent microscope. Biofilm-induced demineralization was conducted again and observed by swept-source optical coherence tomography and confocal laser scanning microscope. The maximum resin penetration depth (PDmax), lesion depth increase (∆LD), frequency of cervical enamel loss and dentinoenamel junction separation length were measured and statistically analyzed. HA120 showed 138.00±49.25 µm PDmax that was significantly larger than PA10 and SEB (p<0.05). SEB created 136.58±64.73 µm coating layers. HA120 and SEB showed significantly lower ∆LD than NC (p<0.05). Resin infiltration with 120s-HCl pretreatment has got a good penetration ability and preventive effect on root caries, however, an additional risk factor of cervical enamel loss was identified.