ゼブラフィッシュ幼虫における歯性フッ素症のグレード分けと定量化．

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Arch. Oral Biol..2016 Oct;70:16-23. S0003-9969(16)30140-6. doi: 10.1016/j.archoralbio.2016.05.021.Epub 2016-05-31.

ゼブラフィッシュ幼虫における歯性フッ素症のグレード分けと定量化．

Grading and quantification of dental fluorosis in zebrafish larva.

Yutao Zhang
Yanli Zhang
Xueni Zheng
Rongchen Xu
Huiming He
Xiaohong Duan

PMID: 27300491 DOI: 10.1016/j.archoralbio.2016.05.021.

抄録

目的:

原始歯における歯科性フッ素症の有病率と重症度は永久歯とは異なる。これまでの歯科フッ素症の動物モデルは、主にラットやマウス、ゼブラフィッシュの幼生を対象としたものが多かった。本研究では、ゼブラフィッシュ幼虫を用いた歯科フッ素症モデルを設定し、フッ化物処理による第一世代の歯の特徴を探ることを目的としている。

OBJECTIVE: The prevalence and severity of dental fluorosis in primary teeth are different from permanent teeth. Previous animal models of dental fluorosis mainly focus on juvenile rats, mice and zebrafish. Our experiment aims to set a dental fluorosis model using zebrafish larva and explore the characteristics of the first generation teeth by fluoride treatment.

材料と方法:

MATERIALS AND METHODS: After the zebrafish eggs were laid, they were exposed to excess fluoride (19ppm, 38ppm and 76ppm) for five days. The morphological characteristics of first generation teeth were examined by H&E staining, whole-mount alizarin red and alcian blue staining, and scanning electron microscope (SEM) technique.

結果:

RESULTS: With whole-mount alizarin red and alcian blue staining, the tooth cusps presented red in normal control. 19ppm and 38ppmm fluoride resulted in extensive red staining from tooth cusps to the lower 1/3 of teeth. 76ppm fluoride caused malformed teeth with uneven red staining. H&E staining showed that excess fluoride caused cystic-like changes in 38ppm and 76ppm groups. SEM revealed the dose dependent pathological changes in zebrafish enameloid with fluoride treatment. Based on SEM findings, we set 0-4 dental fluorosis index (DFI) score to label the severity of dental fluorosis.

結論:

過剰なフッ化物はゼブラフィッシュ幼虫の歯における用量依存性のフッ素症の変化を提示した。この実験で得られたDFIスコアは、用量依存性フッ素症の変化を良い意味で反映しており、今後の歯のフッ素症の研究に役立つと考えられる。

CONCLUSIONS: Excess fluoride presented a dose dependent fluorosis changes in the teeth of zebrafish larva. The DFI scores in our experiment reflect dose dependent fluorosis changes in a good way and will benefit the future research of dental fluorosis.