Neofelis nebulosaの糞便から分離したコレステロール側鎖切断放線菌Gordonia neofelifaecis sp.nov

Gordonia neofelifaecis sp. nov., a cholesterol side-chain-cleaving actinomycete isolated from the faeces of Neofelis nebulosa.

• Yuchang Liu
• Fanglan Ge
• Guiying Chen
• Wei Li
• Pingmei Ma
• Guangxiang Zhang
• Lihua Zeng

抄録

コレステロール側鎖切断性放線菌株AD-6(T)をクラウドヒョウ（Neofelis nebulosa）の新鮮な糞便から分離し、多相分類法を用いて研究した。16S rRNA遺伝子配列解析の結果，本菌はGordonia属の中で異なる亜系統を形成しており，その近縁株はGordonia cholesterolivorans，Gordonia sihwensis，Gordonia hydrophobicaであり，配列類似度はそれぞれ98.2，97.8，97.6"Zs_200A"%であった。AD-6(T)株のgyrB遺伝子配列は、Gordonia属の認識種の型株と77-91"Zs_200A"%の類似度を示し、G. sihwensis、G. hydrophobica、Gordonia hirsutaの型株と最も類似していた（それぞれ91、87、84"Zs_200A"%の類似度）。全細胞脂肪酸分析およびDNA-DNA関連性データの結果から，本菌株は近縁の菌株と容易に区別できた。その結果，AD-6(T)株はGordonia属の新種と考えられ，Gordonia neofelifaecis sp.型はAD-6(T)（=NRRL B-59395(T)=CCTCC AB-209144(T)）である。

A cholesterol side-chain-cleaving bacterial strain, AD-6(T), was isolated from fresh faeces of a clouded leopard (Neofelis nebulosa) and was studied using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis showed that the novel strain formed a distinct subline within the genus Gordonia, its closest neighbours being the type strains of Gordonia cholesterolivorans, Gordonia sihwensis and Gordonia hydrophobica, with sequence similarity values of 98.2, 97.8 and 97.6 %, respectively. The gyrB gene sequence of strain AD-6(T) exhibited similarities of 77-91 % with those of the type strains of recognized species of the genus Gordonia, being most similar to the type strains of G. sihwensis, G. hydrophobica and Gordonia hirsuta (91, 87 and 84 % similarity, respectively). The results of whole-cell fatty acid analyses and DNA-DNA relatedness data readily distinguished the new isolate from its nearest neighbours. Strain AD-6(T) is therefore considered to represent a novel species of the genus Gordonia, for which the name Gordonia neofelifaecis sp. nov. is proposed. The type strain is AD-6(T) (=NRRL B-59395(T)=CCTCC AB-209144(T)).