咬合刺激がラット歯髄の微小血管系に及ぼす影響について

Influence of occlusal stimuli on the microvasculature in rat dental pulp.

• Naoki Shibutani
• Jun Hosomichi
• Yuji Ishida
• Kunimichi Soma

抄録

目的:

咬合機能低下モデルを用いて、咬合刺激が歯髄の血管系に及ぼす影響を検討すること。

OBJECTIVE: To examine the influence of occlusal stimuli on the vasculature in the dental pulp, using an occlusal hypofunction model.

材料と方法:

Sprague-Dawley系雄ラット（7週齢）20匹を2群に分けた。咬合機能低下を起こすために，上顎切歯と下顎切歯に器具を装着した。対照として、未処置のラットを用いた。下顎第一大臼歯の連続水平パラフィン切片を通常の方法で処理した。歯髄内の微小血管を評価するために，各標本の切片をヘマトキシリン・エオジンで染色した．

MATERIALS AND METHODS: Twenty 7-week-old male Sprague-Dawley rats were divided into two groups. To produce occlusal hypofunction, the appliances were attached to the maxillary and mandibular incisors. Untreated rats served as controls. Serial horizontal paraffin sections of the mandibular first molar were processed by conventional methods. To evaluate the microvasculature in the dental pulp, sections of each specimen were stained with hematoxylin-eosin.

結果:

実験群では，歯髄組織内の細動脈は収束して走行しており，その内径は対照群に比べて有意に小さかった．

RESULTS: In the experimental group, the arterioles in the tooth pulp tissue ran convergently, and their inside diameter was significantly smaller than that of the control group.

おわりに:

本研究は、咬合刺激が歯髄の微小血管を通じて歯根膜に影響を与えることを示唆している。

CONCLUSION: This study suggests that occlusal stimuli influence the periodontal ligament throughout the microvasculature of the dental pulp.