異なる根管シーラーの再石灰化能．

Remineralization ability of different root canal sealers.

抄録

目的:

本研究は、異なるシーラー（BioRoot、MTA-FillApex、GuttaFlow-Bioseal）の生体親和性と再石灰化能を対比することを目的とした。

AIMS: This research was designed to contrast the biocompatibility and remineralization ability of different sealers (BioRoot, MTA-FillApex and GuttaFlow-Bioseal).

方法:

この研究では20匹のウサギを使用し、インプラント埋入後3,7,14,28日の観察時間によって無作為に4群に均等に分けた。各ウサギに麻酔を施し、下顎骨腹側左右の皮膚に長さ7cmの切開を加え、頬側歯槽骨の皮質表面に深さ約5mm、直径約2mmの骨空洞を4ヶ所（左側2ヶ所、右側2ヶ所）形成した。The sealers mixed depend on manufactural instructions and immediately insert into the prepared cavities (in the right side the BioRoot and MTA-FillApex were placed while on the left side, GuttaFlow-Bioseal was placed in one cavity and the other cavity was left unfilled as control).The same volume of each sealer was placed in the corresponding cavity using disposable syringes.各観察期間終了後、動物を犠牲にし、試験部位から骨生検を採取し、オリンパス光学顕微鏡を用いて400倍の倍率で組織学的に検査した。

METHOD: Twenty rabbits were used in this study, they were randomly divided into 4 groups equally depending on the observation time"3,7,14, and 28 days" post-implantation. Each rabbit was generally anesthetized,"7cm"long incision was made on the skin of the right and left sides of the ventral aspect of the mandible of each rabbit, 4 bony cavities of approximately"5mm"in depth and"2mm"in diameter (2 cavities on the left side and 2 cavities on the right side of mandible of each rabbit) were made in the cortical surface of the buccal alveolar bone. The sealers mixed depend on manufactural instructions and immediately insert into the prepared cavities (in the right side the BioRoot and MTA-FillApex were placed while on the left side, GuttaFlow-Bioseal was placed in one cavity and the other cavity was left unfilled as control).The same volume of each sealer was placed in the corresponding cavity using disposable syringes. After each observation period, the animals were sacrificed and bone biopsy from the tested area was taken and examined histologically using Olympus light microscopy at"400X"magnification.

結果:

得られたデータは、SPSSソフトウェアバージョン "22 "を用いてノンパラメトリック統計学的検定により分析した。GuttaFlow-Biosealは、すべての評価間隔において炎症組織反応が低いことから、他のグループと比較して優れた生体適合性を示した。一方、バイオルートグループは、ガッタフロー・バイオシールグループに比べ、統計学的に有意ではなかったが、骨伝導性に優れていた。

RESULTS: The obtained data were analyzed through non-parametric statistical tests using SPSS software version"22".Kruskal-Wallis test and Mann - Whitney test were utilized at"0.05"levels of significance to evaluate the results. GuttaFlow-Bioseal displayed excellent biocompatibility in comparison to other groups indicated by low inflammatory tissue reaction at all evaluation intervals. While the BioRoot group represented better osteo-conductivity although statistically not significant than GuttaFlow-Bioseal group.

結論:

バイオルートとガッタフロー・バイオシールは、MTA-FillApexよりも高い骨伝導性と生体適合性を示した。しかし、本研究で使用したシーラーは、いずれも骨組織への適合性が高く、骨修復を促進する可能性がある。

CONCLUSION: BioRoot and GuttaFlow-Bioseal showed higher osteo-conductivity and biocompatibility than MTA-FillApex. However, all sealer used in this study were well tolerated by bone tissue and might accelerate bone repair.