高気圧酸素は自家脂肪移植後の脂肪グラフトの生存率および血管新生を改善する

Hyperbaric Oxygen Improves the Survival and Angiogenesis of Fat Grafts after Autologous Fat Transplantation.

抄録

目的:

現在、自家脂肪移植（Autologous Fat Transplantation：AFT）は、まだグラフトの生存率が低い。AFTのグラフト生存率を上げることは課題である。本研究では、高気圧酸素（HBO）のAFTに対する効果について検討した。

Objective: Currently, autologous fat transplantation (AFT) still has a low graft survival rate. Elevation of the AFT graft survival rate is a challenge. This study investigated the effect of hyperbaric oxygen (HBO) on AFT.

方法:

Methods: Twelve adult male SD rats were randomly divided into two groups after AFT: the control group ( = 6) and the HBO group ( = 6). The rats were killed at 7, 14, and 28 days after transplantation to take the transplanted adipose tissues. The volume and weight of the tissues were detected. The pathological changes in the adipose tissues were observed after H&E staining. Microvessel density and levels of transforming growth factor- (TGF-) , tumor necrosis factor- (TNF-) , and malondialdehyde (MDA) in the transplanted adipose tissues were measured with CD31 immunohistochemical stain, ELISA, and biochemical reagents, respectively. Additionally, the protein expression levels of vascular endothelial growth factor- (VEGF-) A and platelet-derived growth factor- (PDGF) A in the adipose tissues were detected by Western blot.

結果:

HBOは移植脂肪組織の体積と重量を有意に維持し（< 0.01）、移植脂肪組織の病的構造を維持した。HBO療法は移植脂肪組織の炎症因子（TGF-、TNF-）レベルと酸化ストレス（MDA）を減少させる効果があり（ < 0.01 ）、血管新生を促進するCD31とVEGF-A、PDGF-Aなどの血管新生関連因子のレベルを著しく上昇させた（ < 0.01 ）。

Results: HBO significantly preserved the volume and weight of the transplanted adipose tissue ( < 0.01) and maintained the pathological structure of the transplanted adipose tissue. HBO therapy was effective in reducing inflammatory factor (TGF- and TNF-) levels and oxidative stress (MDA) in the transplanted adipose tissue ( < 0.01) and significantly increased the level of CD31 and angiogenesis-related factors including VEGF-A and PDGF-A ( < 0.01) to promote angiogenesis.

結論:

HBO療法は、脂肪移植片の免疫反応を制御し、血管新生を刺激し、最終的にAFT後の生存を促進した。

Conclusion: HBO therapy regulated the immune response of fat grafts, stimulated their angiogenesis, and ultimately promoted their survival after AFT.