[Photothermal effect of nano-copper sulfide against tongue squamous cell carcinoma].

抄録

OBJECTIVE: To investigate the anti-tumor effect of BSA@CuS-PEG nanocomposites on tongue squamous cell carcinoma.

METHODS: Transmission electron microscopy, dynamic light scattering, Zeta potential and ultraviolet absorption spectroscopy were used to characterize the synthesized BSA@CuS-PEG nanocomposite, whose photothermal properties was assessed with near infrared Ⅱ region excitation light (1064 nm). The cytotoxicity of the nanocomposite in Cal27 and SCC9 cells was evaluated using CCK-8 assay, and its effect on cell cycle distribution was analyzed using flow cytometry. The antitumor effect of BSA@CuS-PEG was investigated in a Balb/c mouse model bearing subcutaneous Cal27 tumor xenograft.

RESULTS: The synthesized BSA@CuS-PEG nanocomposite showed a temperature variation (ΔT) of about 30 ℃ under near-infrared (NIR) irradiation (0.5 W/cm2), suggesting its excellent photothermal sensitivity. CCK-8 assay showed that BSA@CuS-PEG had no significant toxicity to tumor cells, but upon NIR irradiation, the nanocomposite produced a significant stronger inhibitory effect on Cal27 and SCC9 cells than free nanocomposites ( < 0.001). Cell cycle analysis showed that compared with free nanocomposites, BSA@CuS-PEG plus NIR irradiation caused more obvious cell cycle arrest at G2/M phase in tongue cancer cells ( < 0.001). In the tumor-bearing mice, BSA@CuS-PEG combined with NIR irradiation produced a significant anti-tumor effect as compared with saline treatment plus NIR irradiation ( < 0.001).

CONCLUSION: The BSA@CuS-PEG nanocomposite shows prominent photothermal properties and good anti-tumor effects both and , and thus provides a promising method for non-invasive early diagnosis and non-surgical treatment of primary tongue cancer.

目的: 探讨BSA@CuS-PEG纳米复合物的制备、表征、功能及其对舌癌的抗肿瘤效应的作用。

方法: 采用透射电镜，动态光散射，Zeta电位分析及紫外吸收光谱分析方法分析BSA@CuS-PEG纳米复合物的表征，利用近红外Ⅱ区激发光（1064 nm）探究其光热性能，同时采用CCK-8实验评估该纳米复合物的细胞毒性，流式细胞学实验分析细胞周期分布水平。最后，构建单侧皮下荷瘤模型探究BSA@CuS-PEG纳米复合物的体内抗肿瘤作用。

结果: 成功制备BSA@CuS-PEG纳米复合物，在0.5 W/cm功率的近红外光照射下其变化温度值（ΔT）约为30 ℃，具有优良的光热性能。CCK8实验表明：单纯BSA@CuS-PEG对肿瘤细胞无明显毒性；而相比于纯材料组，BSA@CuS-PEG联合近红外光照射对Cal27及SCC9舌癌细胞具有显著抑制增殖作用（ < 0.001）。同时细胞周期分析显示：相比于纯材料组，BSA@CuS-PEG联合近红外光照射使Cal27及SCC9舌癌细胞更多被阻滞于G2/M期（ < 0.001）。小鼠单侧皮下荷瘤模型表明：相比于生理盐水联合近红外光照射处理组，BSA@CuS-PEG联合近红外光照射发挥显著的体内抗肿瘤作用（ < 0.001）。

结论: BSA@CuS-PEG纳米复合物具有良好的光热效应，体内外抗肿瘤效应，为舌癌原发灶无创早期诊断和非手术治疗提供一种潜在思路和手段。