歯周病の程度が異なる患者における末梢静脈血中の循環CD34+幹細胞の評価

Evaluation of circulating CD34+ stem cells in peripheral venous blood in patients with varying degrees of periodontal disease.

抄録

はじめに:

歯周病は現代医学の課題であり、現在、幹細胞を用いた治療法に関する研究が進められている。

INTRODUCTION: Periodontal disease presents a challenge for modern medicine, and research on the use of stem cells as a treatment is currently underway.

材料と方法:

この研究では、45人の患者を対象に徹底的な身体検査が行われた。さらに、病歴、歯周病の進行度、血中CRP値の評価が行われた。患者さんは4つのグループに分けられ、歯周病のない患者さんが第1グループ4名、中程度の患者さんが第2グループ8名、進行した患者さんが第3グループ20名、歯のない患者さんが第4グループ13名となりました。各グループに対して，抗体標識したCD34+幹細胞，リンパ球，白血球を用いた幹細胞治療を行った．

MATERIAL AND METHODS: The study included 45 patients who were given a thorough physical examination. Additionally, an evaluation of their medical history of the disease, degree of progression of periodontal disease, and the level of CRP in the blood was carried out. Patients were divided into 4 groups: 4 patients were in the first group with no periodontal disease, 8 patients in the second group with a moderate level, 20 patients in the third group with an advanced level, and 13 patients in the fourth group were toothless. For each group, the use of stem cells as a treatment of antibody-labeled CD34+ stem cells, lymphocytes, and leukocytes was conducted.

結果:

リンパ球に占めるCD34+幹細胞の割合は、中等度群（0.80）、高度群（0.75）、無歯群（0.70）で統計的に有意な正の相関が観察されました。白血球に対するCD34+幹細胞の比率は、無歯顎群（0.92）および進行群（0.91）で統計的に有意であった。CRPの値は、先に述べた患者群で統計的に有意な増加が認められ、CD34+幹細胞の濃度は進行群で最も高いことが示された。

RESULTS: A statistically significant positive correlation was observed in CD34+ stem cells in proportion to lymphocytes in the moderate (0.80), in the advanced (0.75), and in the toothless groups (0.70). The ratio of CD34+ stem cells to leukocytes was statistically significant in the toothless group (0.92) and in the advanced group (0.91). A statistically significant increase was noted in the level of CRP in the previously mentioned groups of patients, and the highest concentration of CD34+ stem cells in the advanced group.

結論:

歯周病が進行した患者群では，CD34+細胞の濃度が最も高いことが確認された．

CONCLUSIONS: The highest concentration of CD34+ cells was observed in the group of patients with advanced periodontal disease.