慢性歯周炎の進行を予測する唾液中miRNAの検出。コホート研究

Detection of Salivary miRNAs That Predict Chronic Periodontitis Progression: A Cohort Study.

抄録

この2年間のコホート研究の目的は、歯周炎の進行を予測する唾液中のマイクロRNA（miRNA）を調査することであった。歯周支持療法を受けた患者 120 例を対象とした．ベースライン時に無刺激の全唾液を採取した．2年後に44名（年齢中央値67.1歳）を追跡調査し，1箇所以上にclinical attachment level（CAL）の進行（ベースライン比3mm以上）または歯周炎の進行による抜歯を認めた進行群（＝22名）と，CALの進行を認めない対照群（＝22名）に分けた．唾液中miRNAのマイクロアレイ解析では、hsa-miR-5571-5p, hsa-miR-17-3p, hsa-let-7f-5p, hsa-miR-4724-3p, hsa-miR-99a-5p, hsa-miR-200a-3p, hsa-miR-28-5p, hsa-miR-320d および hsa-miR-31-5p は fold change値 < 0.5 を示し、 hsa-MiR-28-5p は fold change値 < 0.5 を示した。5または≧2.0を示した（< 0.05）。受信者動作特性曲線解析では、hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p および hsa-miR-320d の曲線下面積は >0.7 で、十分な識別力があることが示された。唾液中のhsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, hsa-miR-320dの発現は慢性歯周炎患者の歯周炎進行と関連した．これらの唾液中miRNAは、歯周炎進行の新たなバイオマーカーとなる可能性があり、そのモニタリングは、歯周炎の新たな診断法や精密医療に貢献する可能性があります。

The aim of this two-year cohort study was to investigate salivary microRNAs (miRNAs) that predict periodontitis progression. A total of 120 patients who underwent supportive periodontal therapy were recruited. Unstimulated whole saliva was collected at baseline. Two years later, 44 patients were followed up (median age, 67.1 years) and divided into two groups: progression group ( = 22), with one or more sites with clinical attachment level (CAL) progression (>3 mm compared with baseline) or tooth extraction due to periodontitis progression; and the control group ( = 22), which did not exhibit CAL progression. In the microarray analysis of salivary miRNAs, hsa-miR-5571-5p, hsa-miR-17-3p, hsa-let-7f-5p, hsa-miR-4724-3p, hsa-miR-99a-5p, hsa-miR-200a-3p, hsa-miR-28-5p, hsa-miR-320d, and hsa-miR-31-5p showed fold change values <0.5 or ≥2.0 in the progression group compared with the control group ( < 0.05). On receiver operating characteristic curve analysis, areas under the curves of hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were >0.7, indicating fair discrimination power. The expressions of salivary hsa-miR-5571-5p, hsa-let-7f-5p, hsa-miR-99a-5p, hsa-miR-28-5p, and hsa-miR-320d were associated with periodontitis progression in patients with chronic periodontitis. These salivary miRNAs may be new biomarkers for progression of periodontitis, and monitoring them may contribute to new diagnostics and precision medicine for periodontitis.