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エナメル質むし歯病変のin vitro脱灰および再石灰化に及ぼすテオブロミンの影響
The effect of theobromine on the in vitro de- and remineralization of enamel carious lesions.
PMID: 34059300 DOI: 10.1016/j.jjodo.2020.100013.
抄録
目的:
エナメル質むし歯の脱灰と再石灰化に及ぼすテオブロミンの影響を、歯垢液と同様の条件で検討する。
OBJECTIVES: This in vitro study investigated the effect of theobromine on the de- and remineralization of enamel carious lesions under plaque fluid-like conditions.
研究方法:
272個のウシのエナメル質標本に初期むし歯病変を形成し、ヌープ表面微小硬さ（SMH）に基づいて16のグループ（n=17）に割り当てた。歯垢液のような条件で、フッ化物（0.2または1ppm）とテオブロミン（0、10、100または200ppm）の存在下、異なるpH値（5.5または7.0）で、病変部を再度脱灰した。再びSMHを測定し、SMH回収率（%SMHr）を算出した。フッ化物，テオブロミン，pHレベルの固定効果に対して三元配置分散分析を行い，各レベル間の差を比較した．
METHODS: Early carious lesions were created in 272 bovine enamel specimens and assigned to sixteen groups (n=17) based on Knoop surface microhardness (SMH). Lesions were demineralized again under plaque fluid-like conditions in the presence of fluoride (0.2 or 1ppm) and theobromine (0; 10; 100 or 200ppm) at different pH values (5.5 or 7.0) in a factorial design. SMH was determined again and percent SMH recovery (%SMHr) calculated. Three-way ANOVA was used for the fixed effects of fluoride, theobromine and pH levels to compare the differences between each level.
結果:
三元交互作用は有意ではなかった（p=0.712）。フッ化物とpHの間の二元交互作用は有意であったが（p＝0.030），フッ化物とテオブロミンの間，およびpHとテオブロミンの間の交互作用は有意ではなかった（それぞれp＝0.478，p＝0.998）．テオブロミンは，いずれの濃度においても%SMHrに影響を与えなかった。0.2ppmのフッ化物をpH5.5で照射した病変は，0.2ppmのフッ化物をpH7.0で照射した病変や1ppmのフッ化物をpH5.5で照射した病変に比べて有意に硬化が少ないという，フッ化物濃度が高いほど，またpHが高いほど硬化が進むという傾向が見られた．
RESULTS: The three-way interaction was not significant (p=0.712). The two-way interaction between fluoride and pH was significant (p=0.030), whereas those between fluoride and theobromine as well as that for pH and theobromine were not (p=0.478 and p=0.998, respectively). Theobromine did not affect %SMHr at any of the tested concentrations. There were trends for the higher fluoride concentration and the higher pH resulting in more rehardening with the lesions exposed to 0.2ppm fluoride at pH 5.5 displaying significantly less rehardening than those exposed to 0.2ppm fluoride at pH of 7.0 and lesions exposed to 1ppm fluoride at pH of 5.5.
結論:
テオブロミンは，様々な濃度と異なるpH値の歯垢液様媒体中に継続的に存在する場合，現在検討されている条件下では，エナメル質むし歯病巣の脱灰や再石灰化に影響を及ぼさないと考えられる．
CONCLUSION: Theobromine, when continuously present in a plaque fluid-like medium at various concentrations and at different pH values, does not affect de- or remineralization of enamel carious lesions under the presently studied conditions.
臨床的意義:
現在入手可能な証拠に基づき、テオブロミンをむし歯予防剤として推奨することはできない。
CLINICAL SIGNIFICANCE: Based on the presently available evidence, theobromine cannot be recommended as an anticaries agent.
Copyright © 2020. Published by Elsevier Ltd.