正方晶鶏卵白リゾチーム結晶における水素/重水素交換挙動は溶液状態に影響される

Hydrogen/deuterium exchange behavior in tetragonal hen egg-white lysozyme crystals affected by solution state.

• Akiko Kita
• Yukio Morimoto

抄録

水素/重水素交換した鶏卵白リゾチームの水素/重水素交換挙動を解明するために、X線と中性子を併用した中性子回折実験を行った。重水素化沈殿試薬を用いて結晶化前に交換された分子からなる中性子仕事用の大きな結晶を得た。水素/重水素交換挙動には、これまでの結晶学的研究やNMR研究との違いがあり、これは結晶中の分子間相互作用や交換期間の長さの違いによるものと考えられます。

Neutron diffraction studies of hydrogen/deuterium-exchanged hen egg-white lysozyme were performed by a joint X-ray and neutron refinement to elucidate the hydrogen/deuterium exchange behavior. Large crystals for neutron work, consisting of molecules that were exchanged before crystallization, were obtained by repeatedly adding protein solution to the crystal batch using deuterated precipitant reagent. There are differences in hydrogen/deuterium exchange behavior compared with previous crystallographic or NMR studies, which could be due to intermolecular interactions in the crystal or to different lengths of exchange period.

© International Union of Crystallography 2020.