2 型糖尿病および高血圧患者における高度糖化最終産物に対する抗体の血清レベル

Serum Levels of Antibodies to Advanced Glycation End Products in Patients with Type 2 Diabetes Mellitus and Hypertension.

• Asparuh Nikolov
• Alexander Blazhev
• Maria Tzekova
• Konstantin Kostov
• Nikola Popovski

抄録

背景と目的:

糖尿病患者の血清中には、高度な糖化末端産物を含むタンパク質が免疫原性が高く、抗高度な糖化末端産物抗体（抗AGEs抗体）が検出された。

BACKGROUND AND AIMS: Proteins containing advanced glycation end products are highly immunogenic and anti-advanced glycation end products antibodies (anti-AGEs antibodies) are found in the sera of diabetics.

材料および方法:

2型糖尿病および動脈性高血圧症患者93名（平均年齢61.4±11.3歳、糖尿病期間9.88±3.12年、高血圧期間9.28±4.98年）の血清中の抗糖化最終生成物抗体の濃度を酵素結合免疫吸着法（ELISA）で測定した。これらの値を、年齢と性別を一致させた42人の対照者の血清抗AGEs抗体と比較した。糖尿病患者を微小血管障害の有無により、第1群（n=67）と第2群（n=26）に分けた。

MATERIALS AND METHODS: Enzyme-linked immunosorbent assay (ELISA) was used for measuring levels of anti-advanced glycation end products antibodies in sera of 93 patients with type 2 diabetes mellitus and arterial hypertension (mean age 61.4±11.3 years, diabetes duration 9.88±3.12 years; hypertension duration 9.28±4.98). These values were compared to serum anti-AGEs antibodies in 42 age and sex matched controls. Diabetics were divided in two groups according to presence or absence of microangiopathy, group 1 (n=67) and group 2 (n=26), respectively.

結果:

2 型糖尿病および動脈性高血圧患者の血清中抗AGEs 抗体レベルは、対照群に比べて統計学的に有意に高かった（1.39±0.39 vs. 1.05±0.32）（p＜0.05）。第1群は健常対照群に比べて有意に高い抗AGEs抗体レベルを示した（1.53±0.14 vs. 1.05±0.32）、（p<0.01）。抗AGEs抗体は微小血管合併症のある患者では合併症のない患者よりも高値であった。抗AGEs抗体は拡張期血圧（r=0.26、p=0.05）、体格指数（r=0.37、p=0.03）と相関していた。抗AGEs抗体陽性者の割合（平均+2SD）は、第1群では第2群に比べて有意に高かった。

RESULTS: Serum levels of anti-AGEs antibodies in patients with type 2 diabetes mellitus and arterial hypertension were statistically significantly higher than those in the control group (1.39±0.39 vs. 1.05±0.32), (p<0.05). Group 1 showed significantly higher levels of anti-AGEs antibodies than those of healthy controls (1.53±0.14 vs. 1.05±0.32), (p<0.01). Anti-AGEs antibodies levels were higher in patients with microvascular complications than these in patients without complications. Anti-AGEs antibodies correlate with diastolic blood pressure (r=0.26, p=0.05) and body mass index (r=0.37, p=0.03). We found significantly higher percentage of positive patients for anti-AGEs antibodies (mean+2SD) in group 1 than in group 2.

結論:

血清中抗AGEs抗体のレベルを決定することは、高血圧患者における後期糖尿病合併症の重症度の早期診断と予後を医師が判断するのに役立つ。

CONCLUSION: Determining the levels of serum anti-AGEs antibodies can help physicians make early diagnosis and prognosis of the severity of late diabetic complications in hypertensive patients.

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