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韓国ソウルの小売食肉からのClostridium perfringensの有病率、毒素型、抗菌薬感受性
Prevalence, toxin-typing, and antimicrobial susceptibility of Clostridium perfringens from retail meats in Seoul, Korea.
PMID: 32619505 DOI: 10.1016/j.anaerobe.2020.102235.
抄録
Clostridium perfringensは、ユビキタス、グラム陽性、胞子形成細菌です。Clostridium perfringensは，多くの小売食肉製品を汚染し，ヒトや家畜の小腸で腸内毒素を産生することで食中毒を引き起こす可能性がある．韓国ソウルの食肉市場から購入した牛肉，鶏肉，豚肉のC. perfringensの有病率，毒素をコードする遺伝子プロファイル，抗菌薬耐性を調査した．C. perfringensは国際標準化機構（International Organization for Standardization 7937）に準拠して検出し，Vitek 2システムを用いて確認した．200の食肉サンプルから合計38株のC. perfringensが分離された（38/200、19％；鶏肉33株、牛肉5株）。α、β、ε、ι、腸管毒素（cpe遺伝子によってコードされる）およびnetBを含む6つの毒素のうち、PCR増幅によりcpa遺伝子のみがすべての分離株から検出された。分離株の抗菌薬耐性を寒天希釈法で評価したところ，アンピシリン（12/38，31.5％），テトラサイクリン（38/38，100％），クロラムフェニコール（26/38，68.4％），メトロニダゾール（13/38，34.2％），イミペネム（27/38，71％）に耐性が認められた。興味深いことに、38株中30株（78.9％）は多剤耐性であり、3種類以上の抗菌薬クラスに耐性を示していた。
Clostridium perfringens is a ubiquitous, Gram-positive, spore-forming bacterium. It can contaminate many types of retail meat products and cause food poisoning by producing enterotoxins in the small intestines of humans and domestic animals. We investigated the prevalence, toxin-encoding gene profile, and antimicrobial resistance of C. perfringens in beef, chicken, and pork meat purchased from retail meat markets in Seoul, Korea. C. perfringens was detected according to the International Organization for Standardization 7937, with some modifications, and confirmed using the Vitek 2 system. In total, 38 C. perfringens strains were isolated from 200 meat samples (38/200, 19%; from thirty-three chicken, and five from beef). Among the six toxins evaluated, including alpha, beta, epsilon, iota, enterotoxin (encoded by the cpe gene), and netB, only the cpa gene was detected in all isolates by PCR amplification. The antimicrobial resistance of the isolates was evaluated using the agar dilution method and resistance to ampicillin (12/38, 31.5%), tetracycline (38/38, 100%), chloramphenicol (26/38, 68.4%), metronidazole (13/38, 34.2%), and imipenem (27/38, 71%) was observed. Interestingly, 30 of the 38 isolates (78.9%) were multiple-drug resistant, showing resistance to more than three different antimicrobial classes.
Copyright © 2020. Published by Elsevier Ltd.