microRNA17の一塩基多型（SNP）rs4284505と歯科フッ素症のリスク

Single nucleotide polymorphism rs4284505 in microRNA17 and risk of dental fluorosis.

• Zerrin Abbasoglu
• Mariana Dalledone
• Letícia M Wambier
• Giovana Pecharki
• Flares Baratto-Filho
• Kesly Mary R Andrades
• Rafaela Scariot
• Paula C Trevilatto
• João A Brancher
• Erika C Küchler

抄録

本研究の目的は，microRNA17（miRNA17）をコード化する遺伝子内の一塩基多型（SNP）rs4284505と歯科性フッ素症（DF）との関連を，ある小児群で評価することである．公衆水道のフッ素化が行われている都市に住む小児を対象とした．DFは、Dean's modified indexを用いて侵食した永久歯で評価した。miRNA17にmiR-SNP rs4284505を選択し、リアルタイムPCRによりジェノタイピングを行った。DFとコントロール間、およびDFの表現型（軽度、中等度、重度）とコントロール間の遺伝子型および対立遺伝子の分布を分析した。研究のために登録された合計527人の子供のうち、383人がDFなし、144人がDFを呈した。ドミナントモデル解析（AA+AG GG）では、miR-SNP rs4284505は中等度のDFと関連しており、GG遺伝子型のキャリアはDFのリスクが2倍以上に増加した（=0.031；オッズ比=2.26、信頼区間95％=1.04-4.73）。Allelic Distributionは中等度のDFでは境界線上の統計的有意性を示し、G alleleのキャリアではDFのリスクが増加した（=.050; Odds Ratio = 1.75, Confidence Interval 95%= 1.00-3.12）。miRNA17のmiR-SNP rs4284505はDFのリスク増加と関連していた。

The aim of this study is to evaluate the association between the single nucleotide polymorphism (SNP) rs4284505 within the gene that codifies microRNA17 (miRNA17) and dental fluorosis (DF) in a group of children. Children living in a city with fluoridation of public water supplies were included. DF was assessed in erupted permanent teeth by Dean's modified index. The miR-SNP rs4284505 was selected in miRNA17 and genotyping was carried out by real-time PCR. Genotype and allelic distributions between DF and control, and between DF phenotypes (mild, moderate and severe) and control were analysed. Among a total of 527 children enrolled for the study, 383 were DF free and 144 presented DF. In the dominant model analysis (AA + AG GG) the miR-SNP rs4284505 was associated with moderate DF, with carriers of the GG genotype having an increased risk of more than two times for DF ( = 0.031; Odds Ratio = 2.26, Confidence Interval 95%= 1.04-4.73). Allelic distribution showed borderline statistical significance for moderate DF with the carriers of G allele having an increased risk for DF ( = .050; Odds Ratio = 1.75, Confidence Interval 95%= 1.00-3.12). The miR-SNP rs4284505 in miRNA17 was associated with an increased risk of DF.