歯内療法中の無菌性作業フィールドの維持

Maintenance of the aseptic working field during endodontic treatment.

抄録

この臨床品質調査の目的は、歯内療法中に無菌作業野が維持されているかどうかを明らかにすることである。 オスロ大学歯内療法科の大学院生が行った歯内療法中に、患者 27 名のラバーダムから細菌試料を採取した。細菌サンプルは，まず作業野の消毒直後に採取し（A），次に水酸化カルシウムセメントによる閉塞またはドレッシングの直前に採取した（B）．細菌の増殖と菌種の検出には，好気性培養法とPCR法を用いた． 培養法では，A，B検体とも培養で陽性となった1例を除き，全検体で陰性であった．PCR法では5例6検体（11％）が陽性であった．ラバーダム消毒後，81％（22/27）の症例で歯内療法中は無菌的作業環境が保たれていた．

The aim of this clinical quality study was to determine whether the aseptic working field is maintained during the endodontic procedure. Bacterial samples were collected from the rubber dam of 27 patients during endodontic treatment performed by postgraduate students at the Department of Endodontics, University of Oslo. A bacterial sample was first obtained immediately after disinfection of the working field (A), and the second sample was collected just before obturation or dressing with calcium hydroxide cement (B). Aerobic cultivation technique and PCR were used for detection of bacterial growth and species. All samples were negative on culturing except in one case, which showed positive results with cultivation in both sample A and B. Specie detected with cultivation technique were . With PCR technique, 6 samples in 5 patients (11%), showed positive results. Species detected with PCR technique were and The present study showed that an aseptic working field was maintained throughout the endodontic procedure in 81% (22/27) of the cases after disinfection of the rubber dam.