超高性能液体クロマトグラフィー-タンデム質量分析法によるコラコリイ・アシーニの馬、牛、羊、豚、ラクダ、鹿の皮の成分の定量

[Determination of the ingredients of horse, ox, sheep, pig, camel and deer skin in colla corii asini by ultra performance liquid chromatography-tandem mass spectrometry].

• Baojian Hang
• Chenying Tian
• Xiao Chen
• Sheng Xing
• Feng Shi
• Jiawei Leng
• Liping Gong

抄録

ロバ、馬、ウシ、ヒツジ、ブタ、ラクダ、シカの皮のコラーゲン配列を比較し、理論的なマーカーペプチドを選択した。マーカーペプチドは、プロテアーゼ切断法と高分解能質量分析法で同定し、UPLC-MS/MSで分析した。馬、牛、羊、豚、ラクダの皮膚のマーカーペプチドが15サンプルから検出された。この方法は簡単で再現性が高く、cola corii asiniの混合皮膚成分の同定に適しており、cola corii asiniの真正性の検出に成功した。

A method was established for detecting the ingredients of horse, ox, sheep, pig, camel and deer skin in colla corii asini using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Theoretical marker peptides were selected by comparing the collagen sequences in the skins of donkeys, horses, oxen, sheep, pigs, camels, and deer.Marker peptides were identified by protease cutting techniques and high resolution mass spectrometry and then analyzed by UPLC-MS/MS.The separation was performed on a UPLC system with a BEH C18 column via the gradient elution of acetonitrile containing 0.1%(v/v) acetic acid and water containing 0.1%(v/v) acetic acid.The marker peptides were identified in the modes of electrospray positive ionization (ESI) and multiple reaction monitoring (MRM).The marker peptides for the horse, ox, sheep, pig and camel skin were detected in 15 samples.The method is simple, highly reproducible, and suitable for the identification of mixed skin ingredients in colla corii asini.It has been successfully used to detect the authenticity of colla corii asini.